Name | Stains-All |
Synonyms | DBTC Stains-All CARBOCYANIN DBTC 3,3'-DIETHYL-9-METHYL-4,5,4',5'-DIBENZOTHIACARBOCYANINE 3,3'-DIETHYL-9-METHYL-4,5,4',5'-DIBENZOTHIACARBOCYANINE BROMIDE 1-ETHYL-2-[3-(1-ETHYLNAPHTHO[1,2-D]THIAZOLIN-2-YLIDENE)-2-METHYLPROPENYL]NAPHTHO[1,2-D]THIAZOLIUM BROMIDE |
CAS | 7423-31-6 |
EINECS | 231-047-7 |
Molecular Formula | C30H27BrN2S2 |
Molar Mass | 559.58 |
Density | 1.3339 (estimate) |
Melting Point | >200℃ |
Water Solubility | Soluble in water. |
Solubility | Soluble in water, ethanol, chloroform |
Appearance | Dull PurplTo Powder |
Color | Dull dark purplish to dark gray |
Maximum wavelength(λmax) | 575 nm |
BRN | 3865213 |
Storage Condition | 2-8℃ |
Sensitive | Light Sensitive |
Physical and Chemical Properties | Biologically active Stains-All, a cationic carbocyanine dye, is studied for calmodulin (CaM) and related calcium binding proteins (CaBP). A convenient probe for the structural features of each calcium binding site. |
In vitro study | Almost all of the proteins found in skeletal muscle extracts, including the Ca 2+ +Mg 2+ -ATPase and the 53,000-Da glycoprotein of the sarcoplasmic reticulum, are stained red or pink with Stains-all. Calsequestrin, the 1 60,000-Da glycoprotein, and 170,000-Da protein are stained blue with Stains-all. The ratio of Stains-all staining (measured at 615 nm) to that of Coomassie blue staining (measured at 575 nm) is 1.3 for calsequestrin, 2.0 for calmodulin, 1.4 for troponin C, and 2.2 for S-100. Therefore, in addition to differentially staining these Ca 2+ -binding proteins blue, Stains-all is a more sensitive stain for these Ca 2+ -binding proteins than is Coomassie blue. |
Safety Description | 24/25 - Avoid contact with skin and eyes. |
WGK Germany | 3 |
FLUKA BRAND F CODES | 3-8-10 |
TSCA | Yes |
HS Code | 29341000 |
Biological applications | Detecting nucleic acid hybridization; identifying microorganisms |
EPA chemical substance information | Naphtho[1,2-d]thiazolium, 1-ethyl-2-[3-(1-ethylnaphtho[1,2-d]thiazol-2(1H)-ylidene)-2-methyl-1-propenyl]-, bromide (7423-31-6) |